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王锡民

王锡民

王锡民

首席研究员

dbswsm@nus.edu.sg

教育背景

1976—1980  南洋理工大学,学士

1980—1983  西弗吉尼亚大学,硕士           

1983—1987  康奈尔大学,博士


研究领域

植物病理学、病毒学、分子生物学、结构生物学、生物技术、纳米技术


研究兴趣

植物病毒分子生物学,对新病毒的发现,病毒的复制和翻译机制,快速灵敏的病毒检测,病毒间的协同作用和病毒与宿主间的相互作用,病毒新基因的功能,利用植物病毒作为载体的蛋白表达,抗病毒转基因植物,病毒的结构生物学和生物信息学,利用植物病毒作为输送药物的纳米材料,RNA干扰。


研究成果

确定两种最流行的兰花病毒CymMV和ORSV的全长序列并产生具有生物活性的cDNA克隆        

从HCRSV和HLSV的polyA片段中发现了两个新颖的开放阅读框

AYVV,CMV和ZYMV的分子表征

成功应用非放射性cRNA探针,聚合酶链反应,毛细管电泳,分子信标和石英晶体微量天平检测植物病毒


代表性论文

[1] Liu HQ, Zhou YJ, Xu QF and Wong SM. (2020). "Selection of DNA aptamers for subcellular localization of RBSDV P10 protein in the midgut of small brown planthoppers by emulsion PCRbased SELEX". Viruses 12, 1239. doi:10.3390/v12111239.

[2] Wu C, Kannan S, Verma CS, Swaminathan K and Wong SM. (2020). "Molecular modeling and interaction between Arabidopsis sulfite oxidase and the GW motif of Turnip crinkle virus coat protein" .Virology 551: 64-74.

[3] Yang X, Das PP, Oppenheimer P, Zhou GH and Wong SM. (2020). "iTRAQ-based protein analysis provides insight into heterologous superinfection exclusion with TMV-43A against CMV in tobacco (Nicotiana benthamiana) plants" .J Proteome 229: 103948. https://doi.org/10.1016/j.jprot.2020.103948.

[4] Guo S and Wong SM. (2020). "Small RNA derived from Tobacco mosaic virus targets a host C2-domain abscisic acid-related (CAR) 7-like protein gene" .Phytopathol. Res. 2: 15. https://doi.org/10.1186/s42483-020-00058-7.

[5] Yang ZT, Wong SM and Yue GH. (2020). "Characterization of GAB3 and its association with NNV resistance in the Asian seabass". Fish & Shellfish Immunol.104: 18-24.

[6] Ren R, Gao J, Lu CQ, Wei YL, Jin JP, Wong SM, Zhu GF and Yang FX. (2020). "Highly efficient protoplast Isolation and transient expression system for functional characterization of flowering related genes in Cymbidium orchids" .Int. J. Mol. Sci. 21, 2264; doi:10.3390/ijms21072264.

[7] Yang ZT, Wang L, Wong SM and Yue GH. (2020). "The HIF1αn gene and its association with hypoxia tolerance in the Asian seabass". Gene 731: 144341. https://doi.org/10.1016/j.gene.2020.144341

[8] Guo S and Wong SM. (2020). "A conserved carboxylesterase inhibits Tobacco mosaic virus (TMV) accumulation in Nicotiana benthamiana plants". Viruses 12, 195; doi:10.3390/v12020195

[9] Macharia M, Das PP, Naqvi NI and Wong SM. (2020). "iTRAQ-based quantitative proteomics reveals a ferroptosis-like programmed cell death in plants infected by a highly virulent tobacco mosaic virus mutant 24A+UPD". Phytopathol. Res. 2:1. https://doi.org/10.1186/s42483-019-0043-5

[10] Wu W, Liu HQ, Dong Y, Zhang Y, Wong SM, Wang CC, Zhou YJ and Xu QF. (2019). "Determination of suitable RT-qPCR reference genes for studies of gene functions in Laodelphax striatellus (Fallén)". Genes. 10.887. doi 10.3390/genes 10110887

[11] Guo S and Wong SM. (2019). "Poly(A) introduced upstream of the upstream pseudoknot domain of Tobacco mosaic virus led to sequence deletion after serial passaging in host plants" .Phytopathol. Res. 1:24.

[12] Macharia MW, Tan WYZ, Das PP, Naqvi NI and Wong SM. (2019). "Proximity-dependent biotinylation screening identifies NbHYPK as a novel interacting partner of ATG8 in plants". BMC Plant Biology 19:326.

[13] Das PP, Macharia MW, Lin QS and Wong SM. (2019). "In planta proximity-dependent biotin identification (BioID) identifies a TMV replication co-chaperone NbSGT1 in the vicinity of 126 kDa replicase". J Proteome 204: 103402


网页链接

https://www.dbs.nus.edu.sg/staffs/wong-sek-man/?cid=59 ?


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